METHOD OF CANCER CELL DETECTION IN PERIPHERAL VENOUS BLOOD IN PATIENTS SUSPECTED OF MALIGNANT GROWTHS Russian patent published in 2011 - IPC G01N33/574 

FIELD: medicine.

SUBSTANCE: for examination, blood is sampled from an elbow vein of an examined patient without regard to food, then blood is dissolved with sodium citrate 1 ml in the blood to sodium citrate relation 8:1. For tumour cell detection, an artificial medium is pressurised in order to enable the examined blood passing through a calibrated filter. The tumour cells are precipitated on the calibrated filter; the precipitate is applied on slides preliminary degreased and cooled for better tumour cell adherence and drying. Thereafter, the smear prints are fixed on the slides with a three-percent Leishman spirit for 2-4 minutes. It is followed by washing with distilled water and staining with an azure eosin mixture in the ratio 3:1 for 6-8 minutes. After staining, the slides are washed with distilled water, air-dried and investigated under microscope.

EFFECT: use of the method allows reducing analysis time, allows instant primary diagnosing in a patient.

6 dwg, 6 ex