METHOD FOR PREPARING BIOLOGICALLY ACTIVE COMPLEX AND BIOLOGICALLY ACTIVE PROTEIN-POLYPEPTIDE COMPLEX Russian patent published in 2011 - IPC A61K35/30 

FIELD: medicine.

SUBSTANCE: declared invention refers to biotechnology, namely to a method for preparing a biologically active complex of embryo brain of hoofed farm animals. The method for preparing the biologically active complex of embryo brain of hoofed farm animals consisting in the fact that fast-frozen embryo brain of preset gestation is defrozen and gradually homogenised in a buffer solution that is combined with simultaneous extraction in the presence of proteolysis inhibitors and non-ionic detergents under certain conditions; the homogenate is separated from undissolved tissue and cell components by centrifugation; the supernatant is filtered; the filtrate is exposed to anion-exchange chromatography; the collected end fractions are desalinated, and preservatives are added; the product is filtered and sterile packed. The biologically active protein-polypeptide complex.

EFFECT: method allows preparing the protein-peptide complex with higher biological activity.

2 cl, 2 tbl, 8 ex