METHOD FOR DETERMINING ANTAGONIST ACTIVITY OF PROBIOTICS FROM SPORE BACTERIA, AGAINST MICOBACTERIA Russian patent published in 2008 - IPC C12Q1/04 C12N1/20 

FIELD: bioengineering.

SUBSTANCE: probiotic is preliminary grown in the Soton liquid medium at 37°C within 5 days and the spores and the spores are couched in two cycles at 37°C within 18-20 hours with the thermal treatment at 132°C within 30 min at the beginning of the first and at the end of the first and the second cycles. 1 ml sterile suspension of the studied probiotic strains is layered on the Levenstein-Jensen solid medium, incubated at 37°C within 24 hours. Then, the tracer strain is intercropped and grown at the temperature and time optimum, with the following determination of the growth blocking index.

EFFECT: provides impartial evaluation of antagonistic activity of spore bacteria against micobacteria.

1 tbl, 4 ex