FIELD: bioengineering.
SUBSTANCE: probiotic is preliminary grown in the Soton liquid medium at 37°C within 5 days and the spores and the spores are couched in two cycles at 37°C within 18-20 hours with the thermal treatment at 132°C within 30 min at the beginning of the first and at the end of the first and the second cycles. 1 ml sterile suspension of the studied probiotic strains is layered on the Levenstein-Jensen solid medium, incubated at 37°C within 24 hours. Then, the tracer strain is intercropped and grown at the temperature and time optimum, with the following determination of the growth blocking index.
EFFECT: provides impartial evaluation of antagonistic activity of spore bacteria against micobacteria.
1 tbl, 4 ex
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DENSE GROWTH MEDIUM FOR CULTIVATION OF PATHOGENIC MYCOBACTERIA AND NOCARDIOFORM RAY FUNGI | 2008 |
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Authors
Dates
2008-07-10—Published
2007-02-13—Filed