FIELD: medicine.
SUBSTANCE: stem cells of an adult human recovered from subcutaneous fat eye tissues are induced to differentiate in insulin-secreting cells in a medium containing cytokines and growth factors, including the B27 additive, fibroblast-2 growth factor, epidermal growth factor, nicotinamide, glucagon-like peptide-1, active A, insulin-like growth factor and betacelluline. For a cultivation process, high-concentrated glucose is replaced for low-concentrated glucose.
EFFECT: invention enables producing cells synthesising considerable amounts of insulin and C-peptide.
3 cl, 8 dwg, 2 tbl, 9 ex
