FIELD: biotechnology.
SUBSTANCE: invention relates to cell biology and biotechnology, specifically to production of insulin. Method includes providing contact of induced human pluripotent stem cells, from which human embryonic stem cells obtained by destroying the embryo are excluded, in vitro with a first cell culture medium containing an agent activating a member of the TGFβ receptor family, selected from the group including Activin A, Activin B, Nodal, GDF-8, GDF-9, GDF-10, GDF-11 and bone morphogenetic protein (BMP). Further, method includes in vitro culturing of said cells in a second medium for growing cells not containing an agent activating a member of the TGFβ receptor family to obtain foregut endoderm cells. Further, the foregut endoderm cells are contacted with an ERBB receptor tyrosine kinase activating agent to obtain a subpopulation of cells, including endocrine cells and non-endocrine cells. Further, said subpopulations mature in vivo, thereby producing insulin-secreting cells.
EFFECT: present invention allows to simplify the production of cells that secrete insulin in response to glucose stimulation.
11 cl, 8 dwg, 12 tbl, 7 ex
