FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology, namely to isolation, maintenance and application of cell cultures. Particularly, it relates to cell compositions obtained from induced pluripotent stem cells. Disclosed are pancreatic cell culture compositions obtained from dedifferentiated reprogrammed pluripotent human stem cells, such as induced pluripotent stem (iPS) cells, for treatment of diabetes. The compositions comprise human pancreatic progenitor cells, including endocrine and non-endocrine subpopulations of cells contained in a nutrient medium containing an epidermal growth factor (EGF) family ligand or a fibroblast growth factor. No less than 30% of said cells are presented by cells of a non-endocrine cell subpopulation.
EFFECT: invention allows to determine and demonstrate detailed properties of differentiation and efficiency of directed differentiation of iPS cells.
22 cl, 8 dwg, 13 tbl, 7 ex
