FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology, namely to isolation, maintenance and application of cell cultures. Particularly, it relates to cell compositions obtained from induced pluripotent stem cells. Disclosed are pancreatic cell culture compositions obtained from dedifferentiated reprogrammed pluripotent human stem cells, such as induced pluripotent stem (iPS) cells, for treatment of diabetes. The compositions comprise human pancreatic progenitor cells, including endocrine and non-endocrine subpopulations of cells contained in a nutrient medium containing an epidermal growth factor (EGF) family ligand or a fibroblast growth factor. No less than 30% of said cells are presented by cells of a non-endocrine cell subpopulation.
EFFECT: invention allows to determine and demonstrate detailed properties of differentiation and efficiency of directed differentiation of iPS cells.
22 cl, 8 dwg, 13 tbl, 7 ex
| Title |
Year |
Author |
Number |
| CELL COMPOSITIONS DERIVED FROM DEDIFFERENTIATED REPROGRAMMED CELLS |
2014 |
- Agulnik Alan D.
- Kelli Oliviya
- Okhi Yuki
- Robins Allan
- Shults Tomas
|
RU2675928C2 |
| IN VITRO DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO PANCREATIC ENDODERMAL CELLS (PEC) AND ENDOCRINE CELLS |
2014 |
|
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2014 |
|
RU2670064C2 |
| IN VITRO DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO PANCREATIC ENDODERMAL CELLS (PEC) AND ENDOCRINE CELLS |
2020 |
- Agulnik, Alan
- D'Amur, Kevin
|
RU2764465C1 |
| METHOD FOR OBTAINING PANCREATIC ENDOCRINE CELLS (VERSIONS) AND METHOD FOR INCREASING OUTPUT OF BETA CELLS |
2013 |
|
RU2684215C2 |
| METHOD FOR OBTAINING CELLS EXPRESSING MARKERS CHARACTERISTIC FOR PANCREATIC ENDOCRINE CELLS |
2013 |
|
RU2658488C2 |
| USE OF EPINEPHRINE LIGANDS TO DIFFERENTIATE PANCREATIC ENDODERM CELLS |
2013 |
|
RU2650813C2 |
| CULTIVATION OF HUMAN EMBRYONIC STEM CELLS IN AN AIR-FLUID INTERACTION ZONE FOR THE PURPOSE OF THEIR DIFFERENTIATION INTO PANCREATIC ENDOCRINE CELLS |
2013 |
|
RU2768963C2 |
| SC-β CELLS AND COMPOSITIONS AND METHODS FOR THEIR CREATION |
2014 |
- Peterson, Kuinn, P.
- Pajliuka, Felitsiya, Dzhej.
- Melton, Duglas, A.
- Millman, Dzheffri, R.
- Segel, Majkl, Saris
- Gyurtler, Mads
|
RU2813705C1 |
| DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO CELLS OF INTESTINAL ENDODERM OF THE MIDDLE INTESTINE |
2017 |
- Rieck, Sebastian
- Rezania, Alireza
|
RU2741114C2 |
