FIELD: medicine.
SUBSTANCE: in order to obtain protein PS-CFP2/TurboYFP_MBP7, constructed is recombinant plasmid DNA PS-CFP2/TurboYFP_MBP7 with size 4916 bp, coding hybrid protein, containing sequence of proteins PS-CFP2 and Turbo YFP, bound with fragment of myelin basic protein 80-104. Composition of plasmid DNA also includes promoter of T5 PHK-polymerase transcription, site of ribosome binding; fragment of DNA plasmid gen of β-lactamase, determining stability of Escherichia coli cells to ampicillin, as genetic marker. Obtained plasmid DNA is used to transform cells of Escherichia coli strain BL21(DE3) to obtain strain-producent of hybrid protein PS-CFP2/TurboYFP_MBP7. In order to obtain protein PS-CFP2/TurboYFP_MBP7 cultivation of strain-producent of Escherichia coli BL21 (DE3)/pQe30_PS-CFP2/TurboYFP_MBP7 is performed, cells are destroyed and target protein is purified by method of affine and gel-filtration chromatography.
EFFECT: invention makes it possible to increase biosensor sensitivity and stability and extend its specificity in respect to pool of catalytic antibodies.
3 cl, 7 dwg, 1 tbl, 3 ex
| Title |
Year |
Author |
Number |
| METHOD OF PRODUCING FUNCTIONALLY ACTIVE RECOMBINANT PROTEIN OF LETHAL FACTOR ANTHRAX (LF), RECOMBINANT PLASMID DNA pETGST-LFmin, ENCODING ACTIVE LF PROTEIN AND STRAIN OF Escherichia coli BL-GSTLFmin, PRODUCING ACTIVE PROTEIN OF LETHAL FACTOR ANTHRAX |
2007 |
- Kolesnikov Aleksandr Vladimirovich
- Zakharova Marija Jur'Evna
- Kozyr' Arina Vladimirovna
- Shemjakin Igor' Georgievich
|
RU2355769C1 |
| WAY OF RECEPTION FUNCTIONALLY ACTIVE RECOMBINANT PROTEIN OF ULCER (LF) LETHAL FACTOR, RECOMBINANT PLASMIDE DNA pETHIS-LF CODING ACTIVE PROTEIN LF; H STRAIN ESCHERICHIA COLI BL-HISLF PRODUCING ACTIVE PROTEIN OF MALIGNANT ANTHRAX LETHAL FACTOR |
2007 |
- Kolesnikov Aleksandr Vladimirovich
- Zakharova Marija Jur'Evna
- Kozyr' Arina Vladimirovna
- Shemjakin Igor' Georgievich
|
RU2361921C1 |
| RECOMBINANT PLASMID DNA pE-His8-TrxL-Ar2 ENCODING FUSION PROTEIN COMPRISING ANTIMICROBIAL SEA ANNELID WORM Arenicola marina PEPTIDE ARENICIN AND STRAIN Escherichia coli BL21(DE3)/pE-His8-TrxL-Ar2 AS PRODUCER OF ARENICIN-CONTAINING FUSION PROTEIN |
2006 |
- Balandin Sergej Vladimirovich
- Kokrjakov Vladimir Nikolaevich
- Ovchinnikova Tat'Jana Vladimirovna
|
RU2316590C1 |
| RECOMBINANT PLASMID DNA pET-KSI-Buf2, CODING HYBRID PROTEIN, CONTAINING ANTIMICROBIAL PEPTIDE BUFORIN-2, STRAIN OF Escherichia coli BL21(DE3)/pET-KSI-Buf2-PRODUCER OF INDICATED PROTEIN AND METHOD OF OBTAINING ANTIMICROBIAL PEPTIDE BUFORIN-2 |
2007 |
- Balandin Sergej Vladimirovich
- Ovchinnikova Tat'Jana Vladimirovna
|
RU2347811C1 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli |
2013 |
- Petrovskaja Lada Evgen'Evna
- Shingarova Ljudmila Nikolaevna
- Gapizov Sultan Shakhbanovich
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Jakimov Sergej Aleksandrovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2527171C1 |
| PLASMID VECTOR pE-Lc-LTP, Escherichia coli BACTERIUM STRAIN FOR EXPRESSION OF LIPID-TRANSPORTING LENTIL PROTEINS Lens culinaris AND METHOD FOR PRODUCING SAID PROTEINS |
2009 |
- Ovchinnikova Tat'Jana Vladimirovna
- Finkina Ekaterina Ivanovna
- Balandin Sergej Vladimirovich
|
RU2415940C1 |
| RECOMBINANT PLASMID DNA pMALTEV-legumain, ENCODING POLYPEPTIDE HAVING ANTIGENIC PROPERTIES OF PROTEIN LEGUMAIN Opisthorchis felineus, AND STRAIN E.coli BL 21(DE3)pLysS-pMALTEV-legumain - PRODUCENT OF RECOMBINANT POLYPEPTIDE HAVING ANTIGENIC PROPERTIES OF PROTEIN LEGUMAIN Opisthorchis felineus |
2012 |
- L'Vova Marija Nikolaevna
- Mordvinov Vjacheslav Alekseevich
- Ponomareva Evgenija Pavlovna
- Sivkov Anton Jur'Evich
- Razumov Ivan Alekseevich
- Katokhin Aleksej Vadimovich
|
RU2496876C1 |
| RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE |
2015 |
- Petrovskaya Lada Evgenevna
- Shingarova Lyudmila Nikolaevna
- Kryukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Zlobinov Aleksandr Vladimirovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2606014C1 |
| RECOMBINANT PLASMID DNA pET-32a, CODING GEN LIGNAD-BINDING DOMAIN OF RECEPTOR II TYPE OF HUMAN TRANSFORMING GROWTH FACTOR-β (TβRII), STRAIN OF BACTERIA Escherichia coli - PRODUCENT OF FUSION PROTEIN THIOREDOXIN/TβRII AND METHOD OF RENATURATION AND PURIFICATION OF TARGET PROTEIN TβRII |
2009 |
- Gasparjan Marine Ehduardovna
- Elistratov Pavel Alekseevich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2413769C1 |
| RECOMBINANT PLASMID DNA OF PTRX-TEVRS-PTH CODING A HYBRID PROTEIN CAPABLE OF PROTEOLYTIC SPLITTING TO FORM A FRAGMENT OF ENDOGENOUS HUMAN PARATHYROID HORMONE (1-34), AN ESCHERICHIA COLI BL21(DE3)/PTRX-TEVRS-PTH STRAIN - PRODUCER OF SAID PROTEIN AND A METHOD OF PRODUCING RECOMBINANT PTH (1-34) |
2019 |
- Myagkikh Igor Valentinovich
- Stepanenko Vasilij Nikolaevich
- Puchkov Ilya Aleksandrovich
- Novozhilov Nikolaj Mikhajlovich
- Makarov Dmitrij Aleksandrovich
- Sokolova Irina Vladimirovna
- Vorobeva Tatyana Vladimirovna
- Levandovskaya Kristina Georgievna
- Pavlenko Daniil Mikhajlovich
- Zinchenko Aleksej Alekseevich
- Miroshnikov Anatolij Ivanovich
- Kostromina Mariya Andreevna
- Esipov Roman Stanislavovich
|
RU2700452C1 |
