FIELD: medicine.
SUBSTANCE: read values of the conductometric microparticle counter PICOSCALE are subtracted from each other at various voltage thresholds in increments 0.5V; absolute red blood cell count with various volumes is calculated by formula: Ea=En-En+1, where Ea is absolute red blood cell count with various volumes, En is red blood cell count of volume exceeding a discrimination level, i.e. reading with a minimum cell volume detected by the device at the preset voltage threshold, En+1 is a following value of red blood cell count of volume exceeding a discrimination level in the following position of a voltage adjuster, and an red blood cell percentage is determined by the relation of absolute red blood cell count with various volumes to total red blood cell (RBC) count.
EFFECT: method allows detecting pathological changes in red blood cell population, and providing higher accuracy of cytometry and reducing time of the procedure.
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Authors
Dates
2011-10-20—Published
2010-03-05—Filed