Show metadata Hide metadata

(19)

(11)

2 432 396

(13)

(51)

IPC

C12N9/68(2006-01-01)

C12N15/52(2006-01-01)

C12N15/63(2006-01-01)

C12N1/21(2006-01-01)

(21) (22)

Application

2009143096/10, 2009-11-24

(24)

Start date

2009-11-24

(22)

Actual filing date

2009-11-24

(45)

Published

2011-10-27

(72)

Inventor

Bibilashvili Robert ShalvovichBelogurov Anatolij AleksandrovichGurskij Jaroslav GeorgievichMinashkin Mikhail MikhajlovichSkamrov Andrej ViktorovichSkrypina Natalija AlekseevnaFeoktistova Evgenija Sergeevna

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Kardiologicheskij Nauchno-Proizvodstvennyj Kompleks" Ministerstva Zdravookhranenija I Sotsial'Nogo Razvitija Rossijskoj Federatsii Minzdravsotsrazvitija Rossii)Rossijskaja Federatsija, Ot Imeni Kotoroj Vystupaet Ministerstvo Obrazovanija I Nauki Rossijskoj Federatsii

RECOMBINANT POLYPEPTIDE WITH HUMAN PLASMINOGEN PROPERTIES TO CONVERT INTO PLASMIN IF ACTIVATED WHICH CATALYSES FIBRIN SPLITTING, DNA FRAGMENT CODING POLYPEPTIDE, RECOMBINANT PLASMID DNA FOR POLYPEPTIDE EXPRESSION AND TRANSFORMED Escherichia coli CELL - POLYPEPTIDE PRODUCER Russian patent published in 2011 - IPC C12N9/68 C12N15/52 C12N15/63 C12N1/21

Abstract RU 2432396 C2

FIELD: medicine.

SUBSTANCE: genetically engineered technique is applied to produce in Escherichia coli cells the recombinant polypeptides with human plasminogen properties containing a catalytic plasminogen domain, 5th and 4th plasminogen kringle domains, and a modified fragment of an amino acid sequence preceding to 4 plasminogen kringle domains.

EFFECT: invention allows producing the polypeptides of high fibrinolytic human plaminogen activity and yield 40-60 mg per 1 l of the culture in expression in Escherichia coli cells.

7 cl, 1 dwg, 3 ex

Similar patents RU2432396C2

Title	Year	Author	Number
RECOMBINANT POLYPEPTIDE WITH HUMAN PLASMINOGEN PROPERTIES TO CONVERT INTO PLASMIN IF ACTIVATED WHICH CATALYSES FIBRIN SPLITTING, DNA FRAGMENT CODING POLYPEPTIDE, RECOMBINANT PLASMID DNA FOR POLYPEPTIDE EXPRESSION AND TRANSFORMED Escherichia coli CELL - POLYPEPTIDE PRODUCER	2009	Bibilashvili Robert Shalvovich Belogurov Anatolij Aleksandrovich Gurskij Jaroslav Georgievich Minashkin Mikhail Mikhajlovich Skamrov Andrej Viktorovich Skrypina Natalija Alekseevna Feoktistova Evgenija Sergeevna	RU2432397C2
METHOD TO PRODUCE RECOMBINANT ANTI-ANGIOGENIC POLYPEPTIDE	2012	Glybochko Petr Vital'Evich Svistunov Andrej Alekseevich Nikolenko Vladimir Nikolaevich Lutsenko Sergej Viktorovich Fel'Dman Natalija Borisovna Solov'Ev Andrej Ivanovich	RU2488635C1
PLASMID FOR EXPRESSION IN BACTERIA CELLS OF GENUS Escherichia OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (TPA) (RETEPLASE), BACTERIA BELONGING TO GENUS Escherichia, - PRODUCER OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE) OR [-1] METHIONYL - FRAGMENT OF HUMAN TPA (RETEPLASE), PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE), METHOD OF PRODUCTION OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE)	2011	Orlova Nadezhda Aleksandrovna Vorob'Ev Ivan Ivanovich	RU2495933C2
TWO-COMPONENT PHARMACEUTICAL COMPOSITION HAVING FIBRINOLYTIC EFFECT	2020	Bibilashvili Robert Shalvovich Belyanko Tatyana Igorevna Gurskij Yaroslav Georgievich Skrypina Nataliya Alekseevna	RU2765043C1
PLASMINOGEN UROKINASE TYPE MODIFIED ACTIVATOR, DNA SEQUENCE, RECOMBINANT PLASMID, STRAIN-PRODUCER, METHOD FOR PREPARING PLASMINOGEN UROKINASE TYPE MODIFIED ACTIVATOR AND PHARMACEUTICAL COMPOSITION ELICITING THROMBOLYTIC EFFECT	2001	Gurskij Ja.G. Belogurov A.A. Bibilashvili R.Sh. Grigor'Eva N.V. Del'Ver E.P. Minashkin M.M.	RU2247777C2
RECOMBINANT PLASMID DNA pBK415 CODING POLYPEPTIDE OF RECOMBINANT TISSULAR ACTIVATOR OF PLASMINOGEN OF HUMAN BEING, CELL LINE Cricetulus griseus CHO 1F8-PRODUCER OF RECOMBINANT TISSULAR ACTIVATOR OF PLASMINOGEN OF HUMAN BEING, AND METHOD FOR OBTAINING AND SEPARATION OF POLYPEPTIDE HAVING ACTIVITY OF TISSULAR ACTIVATOR OF PLASMINOGEN	2012	Grigor'Eva Ol'Ga Vasil'Evna Zaval'Nyj Mikhail Aleksandrovich Karpov Andrej Pavlovich Petrov Andrej Vladimirovich Fabrichnyj Igor' Pavlovich Shuster Aleksandr Mikhajlovich	RU2500817C1
RECOMBINANT TISSUE ACTIVATOR OF PLASMINOGEN AND METHOD FOR ITS PRODUCTION	1988	Jasusi Kavauti[Jp] Tosijuki Takemoto[Jp] Makoto Takajama[Jp] Masami Ekota[Jp] Masao Kato[Jp] Kimio Katsuta[Jp] Khirosi Gusima[Jp]	RU2107727C1
RECOMBINANT HYBRID INHIBITOR OF ANGIOGENESIS, AND ITS PRODUCTION METHOD	2012	Glybochko Petr Vital'Evich Svistunov Andrej Alekseevich Nikolenko Vladimir Nikolaevich Lutsenko Sergej Viktorovich Fel'Dman Natalija Borisovna Solov'Ev Andrej Ivanovich	RU2499802C1
RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli	2013	Petrovskaja Lada Evgen'Evna Shingarova Ljudmila Nikolaevna Gapizov Sultan Shakhbanovich Krjukova Elena Aleksandrovna Boldyreva Elena Filippovna Jakimov Sergej Aleksandrovich Dolgikh Dmitrij Aleksandrovich Kirpichnikov Mikhail Petrovich	RU2527171C1
RECOMBINANT HYBRID POLYPEPTIDE, CAPABLE OF INHIBITING HUMAN ENDOTHELIAL CELL PROLIFERATION IN VITRO, AND METHOD FOR PRODUCTION THEREOF	2011	Svistunov Andrej Alekseevich Lutsenko Sergej Viktorovich Fel'Dman Natalija Borisovna Solov'Ev Andrej Ivanovich	RU2465283C1

RU 2 432 396 C2

Authors

Bibilashvili Robert Shalvovich

Belogurov Anatolij Aleksandrovich

Gurskij Jaroslav Georgievich

Minashkin Mikhail Mikhajlovich

Skamrov Andrej Viktorovich

Skrypina Natalija Alekseevna

Feoktistova Evgenija Sergeevna

Dates

2011-10-27—Published

2009-11-24—Filed