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2 432 397

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IPC

C12N9/68(2006-01-01)

C12N15/52(2006-01-01)

C12N15/63(2006-01-01)

C12N1/21(2006-01-01)

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Application

2009143097/10, 2009-11-24

(24)

Start date

2009-11-24

(22)

Actual filing date

2009-11-24

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Published

2011-10-27

(72)

Inventor

Bibilashvili Robert ShalvovichBelogurov Anatolij AleksandrovichGurskij Jaroslav GeorgievichMinashkin Mikhail MikhajlovichSkamrov Andrej ViktorovichSkrypina Natalija AlekseevnaFeoktistova Evgenija Sergeevna

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Kardiologicheskij Nauchno-Proizvodstvennyj Kompleks" Ministerstva Zdravookhranenija I Sotsial'Nogo Razvitija Rossijskoj Federatsii Minzdravsotsrazvitija Rossii)Rossijskaja Federatsija, Ot Imeni Kotoroj Vystupaet Ministerstvo Obrazovanija I Nauki Rossijskoj Federatsii

RECOMBINANT POLYPEPTIDE WITH HUMAN PLASMINOGEN PROPERTIES TO CONVERT INTO PLASMIN IF ACTIVATED WHICH CATALYSES FIBRIN SPLITTING, DNA FRAGMENT CODING POLYPEPTIDE, RECOMBINANT PLASMID DNA FOR POLYPEPTIDE EXPRESSION AND TRANSFORMED Escherichia coli CELL - POLYPEPTIDE PRODUCER Russian patent published in 2011 - IPC C12N9/68 C12N15/52 C12N15/63 C12N1/21

Abstract RU 2432397 C2

FIELD: medicine.

SUBSTANCE: genetically engineered technique is applied to produce in Escherichia coli cells the recombinant polypeptides with human plasminogen properties, containing a catalytic plasminogen domain, 5th plasminogen kringle domains, and a modified fragment of an amino acid sequence preceding to the 5th plasminogen kringle domain.

EFFECT: invention allows producing the polypeptides of high fibrinolytic human plaminogen activity and high yield in expression in Escherichia coli cells.

7 cl, 1 dwg, 11 ex

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RU 2 432 397 C2

Authors

Bibilashvili Robert Shalvovich

Belogurov Anatolij Aleksandrovich

Gurskij Jaroslav Georgievich

Minashkin Mikhail Mikhajlovich

Skamrov Andrej Viktorovich

Skrypina Natalija Alekseevna

Feoktistova Evgenija Sergeevna

Dates

2011-10-27—Published

2009-11-24—Filed