FIELD: chemistry.
SUBSTANCE: method involves: (a) culturing E.coli cells containing polypeptide-coding nucleic acid in a culture medium into which transportable organophosphate is fed, said organophosphate being selected from a group comprising alpha-glycerophosphate, beta-glycerophosphate, glycerol-3-phosphate, and a mixture of glycerol-2-phosphate and glycerol-3-phosphate, such that nucleic acid is expressed, and (b) extracting polypeptide from cells, wherein during the culturing step, inorganic phosphate is added to the culture medium.
EFFECT: improved heterologous protein expression and high output of the end product.
29 cl, 10 dwg, 1 tbl, 5 ex
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