HOLDER FOR SOFT BIOLOGICAL TISSUE SAMPLE TO MICROTOME Russian patent published in 2011 - IPC G01N1/06 

FIELD: mechanics.

SUBSTANCE: holder includes housing with surface on which there arranged is investigated object and pressure sponge containing a set of parallel located needles. Housing is made so that surface on which the investigated object is located is made in the form of flat base with holes, along the perimetre of which a guide is fixed on the side opposite to investigated object. Inside the guide there arranged is pressure sponge protruding beyond its limits and copying the inner shape of the guide with possibility of coaxial movement of pressure sponge inside guide. At that, pressure sponge is made in the form of upper part connected as one piece to lower part. In upper part there rigidly fixed are needles protruding beyond the limits of upper part towards the housing so that needles can be arranged in holes of the housing. At that, needles are located along the perimetre of upper part of pressure sponge at equal distance between each other, and holes of housing coincide with axis of needles the protruding pointed tips of which have chamfer opposite direction of microtome blade stroke. In lower part of pressure sponge on the side of microtome blade stroke there is longitudinal slot and transverse cavity interconnected with it. Spring-loaded limit stop made in the form of plate having corrugation in upper part of the plate and equal to the length of the guide is installed in longitudinal slot. At that, corrugation is made on the plate side facing inner side of the housing guide with possibility of interaction of corrugation with corrugation made on surface of the guide adjacent to plate of spring-loaded limit stop. Plate has bent end arranged in transverse cavity. Besides, length of upper and lower parts of pressure sponge, which are connected as one piece, is equal to sum of length of the guide at the position of upper part when it is combined with flat base of housing, and protruding beyond the limits of the guide of length of pressure sponge, which is enough to ensure possible arrangement of handles on side surfaces of pressure sponge. Push-button having the possibility of sliding in groove of microtome rod is fixed on the level of arrangement of handles on outer surface of spring-loaded limit stop plate.

EFFECT: lower deformation degree of soft biological tissue during sampling.

6 dwg