FIELD: medicine.
SUBSTANCE: what is described is an oligonucleotide probe with complementary terminal "molecular beacon" sequences: 5'-(FAM)- GCCTCGGTGAGTCGGCCCCTAAGGCGAGGC-(RTQl)-3'. FAM is carboxyfluorescein, a fluorescent dye of absorption wave length 492 nm, and fluorescence wave length is 520 nm. RTQ1 is a fluorescence absorber with the absorbing range 470-570 nm. The invention provides high-sensitivity and specificity identification of glanders and melioidosis agents in a relatively short time in a biological material and environmental objects.
EFFECT: invention can be used in medicine for detection of a genetic material of glanders and melioidosis agents Burkholderia mallei and Burkholderia pseudomallei in samples both for diagnostics in practical public health service and Federal Service for Supervision of Consumer Rights and Human Welfare, and for scientific research.
3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| FLUORESCENT-MARKED OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF GLANDERS AND MELIOIDOSIS AGENTS Bpseudomallei AND Bmallei | 2010 |
|
RU2435861C1 |
| OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS B. pseudomallei AND B. mallei | 2010 |
|
RU2439159C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR IDENTIFICATION OF Burkholderia mallei AND ITS DIFFERENTIATION FROM Burkholderia pseudomallei | 2014 |
|
RU2551208C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR IDENTIFICATION OF Burkholderia pseudomallei | 2014 |
|
RU2556810C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES AND METHOD FOR DETECTING DNA OF AGENTS OF GLANDERS AND MELIOIDOSIS BY PCR METHOD WITH PRODUCT DETECTION IN REAL TIME | 2019 |
|
RU2738358C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENT-LABELED PROBE FOR IDENTIFYING RNAS OF BURKHOLDERIA MALLEI SAP EXCRETION AND BURKHOLDERIA PSEUDOMALLEI MELIOIDOSIS BASED ON TRANSCRIPTION AMPLIFICATION (NASBA) IN REAL TIME MODE | 2018 |
|
RU2699180C1 |
| OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2474617C1 |
| OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2474616C1 |
| OLIGONUCLEOTIDE PRIMERS FOR B.mallei GENETIC TYPING BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2478715C1 |
| OLIGONUCLEOTIDE PRIMERS FOR B.mallei GENETIC TYPING BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2478714C1 |
