FIELD: biotechnology.
SUBSTANCE: invention relates to a set of oligonucleotide primers and fluorescently-labelled probe for identification of Burkholderia pseudomallei and differentiation of the actinobacillus mallei by the method of polymerase chain reaction with fluorescence detection.
EFFECT: invention enables in a short time with high sensitivity and specificity to detect the melioidosis agent and differentiate it from the actinobacillus mallei in samples of pure cultures and biological material.
1 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR IDENTIFICATION OF Burkholderia mallei AND ITS DIFFERENTIATION FROM Burkholderia pseudomallei | 2014 |
|
RU2551208C1 |
| FLUORESCENT-MARKED OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF GLANDERS AND MELIOIDOSIS AGENTS Bpseudomallei AND Bmallei | 2010 |
|
RU2435861C1 |
| FLUORESCENT-MARKED OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF GLANDERS AND MELIOIDOSIS AGENTS Bpseudomallei AND Bmallei | 2010 |
|
RU2435860C1 |
| OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS B. pseudomallei AND B. mallei | 2010 |
|
RU2439159C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES AND METHOD FOR DETECTING DNA OF AGENTS OF GLANDERS AND MELIOIDOSIS BY PCR METHOD WITH PRODUCT DETECTION IN REAL TIME | 2019 |
|
RU2738358C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENT-LABELED PROBE FOR IDENTIFYING RNAS OF BURKHOLDERIA MALLEI SAP EXCRETION AND BURKHOLDERIA PSEUDOMALLEI MELIOIDOSIS BASED ON TRANSCRIPTION AMPLIFICATION (NASBA) IN REAL TIME MODE | 2018 |
|
RU2699180C1 |
| SET OF FLUORESCENTLY LABELLED OLIGONUCLEOTIDE PROBES FOR TYPING STRAINS OF Burkholderia mallei BY METHOD OF AMPLIFICATION OF DIFFERENTIATING DNA FRAGMENTS | 2014 |
|
RU2551227C1 |
| OLIGONUCLEOTIDE PRIMERS FOR β LACTAMASE GENE DETECTON AND TYPING OF PATHOGENIC BURKHOLDERIA | 2011 |
|
RU2474614C1 |
| OLIGONUCLEOTIDE PRIMERS BmVAT5-Ch2s/BmVAT5-Ch2as FOR DETECTING BMAA0107 DIFFERENTIATION FRAGMENT OF EQUINIA AGENT STRAINS | 2012 |
|
RU2474620C1 |
| OLIGONUCLEOTIDE PRIMERS FOR B.mallei GENETIC TYPING BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2478715C1 |
