FIELD: medicine.
SUBSTANCE: method of human regulatory CD4+CD25+FOXP3+ T-cell enrichment ex vivo consists in recovery of CD4+ T-cell population from patient's peripheral blood and cultivation thereof in a growth medium containing 5-10 % autologous serum, 10-1000 units/ml of interleukin-2, 0.5-10 mcg/ml of monoclonal human CD3-antigen antibodies, 0.1-10 mcg/ml of monoclonal human CD28-antigen antibodies and 1-50 ng/ml of the transforming growth factor-beta1 per 1 ml of the growth medium.
EFFECT: invention allows producing the regulatory T-cell enriched autologous lymphocyte population suitable for treating the diseases characterised by prominently decreased T-cell count or functional activity.
10 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING POPULATION OF HUMAN CD4+CD25+Foxp3+ T-LYMPHOCYTES ex vivo, DISEASE TREATMENT METHOD | 2008 |
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RU2391401C2 |
| METHOD OF THERAPY OF REMITTENT MULTIPLE SCLEROSIS | 2012 |
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| METHOD FOR OBTAINING AUTOLOGOUS REGULATORY T-LYMPHOCYTES BY EX VIVO CULTIVATION IN PRESENCE OF HUMAN CHORIONIC GONADOTROPIN | 2022 |
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| DIAGNOSTIC TECHNIQUE FOR IMMUNOLOGICAL INFERTILITY | 2014 |
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RU2553341C1 |
| MONOCYTIC SELF-SPECIFIC CELLS, STIMULATING AUTOTOLERANCE, AND THEIR USE IN PHARMACEUTIAL PREPARATIONS | 2004 |
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| CELL LINE OF HUMAN KIDNEY CANCER IBGVAT R 27 | 2014 |
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RU2573938C1 |
| METHOD OF OBTAINING CELLS OF MONOCYTE ORIGIN INDUCING TRANSPLANT ACCEPTANCE, METHOD OF OBTAINING PHARMACEUTICAL COMPOSITION SUPPRESSING TRANSPLANT REJECTION REACTION, CELLS OF MONOCYTE ORIGIN INDUCING TRANSPLANT ACCEPTANCE, CELL MEDICINE FOR TRANSPLANT ACCEPTANCE INDUCTION, PHARMACEUTICAL COMPOSITION SUPPRESSING TRANSPLANT REJECTION REACTION, APPLICATION OF CELLS INDUCING TRANSPLANT ACCEPTANCE (VERSIONS),METHOD OF OBTAINING AND/OR REPRODUCTION OF REGULATORY T-LYMPHOCYTE, HYBRIDOMA CELL LINE, ANTIBODY AND ANTIBODY APPLICATION | 2003 |
|
RU2370535C2 |
| CELL LINE OF HUMAN KIDNEY CANCER IBGVAT R 6 | 2014 |
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RU2573940C1 |
| METHOD FOR INCREASING LEVEL OF AUTOLOGIC T-REGULATORY LYMPHOCYTES IN HUMAN BLOOD CELL CULTURES | 2023 |
|
RU2812053C1 |
| COMPOSITIONS TO TREAT INFLAMMATORY BOWEL CONDITION | 2008 |
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RU2495121C2 |
