FIELD: medicine.
SUBSTANCE: to 1 part of water solution, which contains 1 mg/ml of plasmid DNA, added are 10 parts of water and 1 part of suspension, containing 4 g/ml of superparamagnetic parts of magnetite. After that into obtained solution added is 1 part of buffer solution with pH 7.5, 2 parts of 0.005 M solution of ribonucleotidetriphpsphates, 2 parts of 0.1 M of dithiotreitole, 1 part of solution, containing 2 mg/ml of bovine serum albumin, 1 part of solution, which contains 10 units of RNAase inhibitor and 1 part of solution, which contains 10 units of PNA-polymerase of T3 or T7 or SP6 bacteriophage. Obtained mixture is incubated for not less than 1 hour at temperature +37°C and placed into magnetic field with intensity not less than 0.2 T for 1-3 minutes. After that water phase, containing target RNA is decanted.
EFFECT: RNA, obtained by claimed method, is of high quality and is suitable for molecular-biological studies of gene structure and functions, as well as for diagnostic and therapeutic purposes in medical practice.
2 cl, 1 dwg, 1 ex
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