FIELD: biotechnology.
SUBSTANCE: pET-15b_T3_RL plasmid DNA is constructed which provides synthesis of recombinant fusion protein consisting of a tumor-specific peptide and an antitumor peptide RL2. The recombinant fusion protein isolated from Escherichia coli cells transformed with pET-15b_T3_RL plasmid, has a molecular weight of 15.9 kDa, consists of methionine residue, tumor specific peptide YTYDPWLIFPAN, glycine GGGS spacer, and a recombinant lactaptin RL2 analogue.
EFFECT: production of a protein providing cytotoxic activity against cancer cells in culture and targeted properties for tumor tissue.
2 cl, 6 dwg, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID pET-15b_T1_RL DNA PROVIDING SYNTHESIS OF RECOMBINANT FUSION PROTEIN CONSISTING OF TUMOR-SPECIFIC PEPTIDES AND ANTITUMOR PEPTIDE RL2, AND RECOMBINANT FUSION PROTEIN POSSESSING CYTOTOXIC ACTIVITY AGAINST CANCER CELLS AND TARGETED PROPERTIES AGAINST TUMOR TISSUE | 2016 |
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RU2619050C1 |
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| METHOD FOR EXTRACTING AND PURIFYING RECOMBINANT PROTEIN, AN ANALOGUE OF A HUMAN KAPPA-CASEIN FRAGMENT HAVING CYTOTOXIC ACTIVITY TOWARDS HUMAN CANCER CELLS | 2018 |
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RU2621861C1 |
| RECOMBINANT OF VV-GMCSF / LACT-DGF VIRUS OF OSVOVICINE, OF ONLY-COLIC ACTIVITY AND PRODUCING SECRETABLE CHIMERIC WHITE, CONTAINING FROM GRANULOCYTE-MACROPHAGAL COLONISTIMULATING HUMAN FACTOR AND ONCOTOXIC PROTEIN OF LACTAPTIN | 2016 |
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| PEPTIDE WITH ANTITUMOR ACTIVITY | 2017 |
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RU2730657C1 |
