METHOD FOR PRODUCING ASSOCIATED COLIBACILLOSIS, STREPTOCOCCOSIS AND RABBIT VIRAL HEMORRHAGIC DISEASE VACCINE Russian patent published in 2012 - IPC C12N1/20 A61K39/295 

FIELD: medicine.

SUBSTANCE: affected organs of dead rabbits from a local epizootic centre are sampled to prepare a suspension, and differential-diagnostic mediums are inoculated; pure-growth agents are recovered; separately, E. coli, Str. pneumoniae cultures are grown in a meat-peptone broth with glucose added to the concentration of 0.2% and titre 4-5 billion microbial cells in 1 cm³; then rabbits are infected with a hemorrhagic disease virus of the activity not less than 10^3.0 LD₅₀/cm³, and liver of dead rabbits is used to prepare 10-15% suspension; thereafter the agent cultures are separately inactivated with formalin to the final concentration no more than 0.4% at temperature 37°C for 4 days or less; then the mixed inactivated cultures are added with an adjuvant presented by aluminium hydroxide gel in the amount of 20 vol %; the end product is thoroughly mixed before packing.

EFFECT: invention provides producing a harmless, high-immunogenic storage-stable vaccine.

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