METHOD FOR PRODUCING ASSOCIATED SALMONELLOSIS, ESCHERICHIOSIS AND RABBIT VIRAL HEMORRHAGIC DISEASE VACCINE Russian patent published in 2013 - IPC A61K39/116 A61K39/112 A61K39/108 A61K39/12 

FIELD: medicine.

SUBSTANCE: invention refers to veterinary medicine, and concerns a method for producing an associated salmonellosis, escherichiosis and rabbit viral hemorrhagic disease vaccine. Implement the method involves sampling the affected organs from fallen rabbits on the local epidemical block to prepare a suspension that is followed by inoculation of differential diagnostic media. Pure cultures of pathogens are recovered. The culture Salmonella typhimurium, Escherichia coli is separately grown in meat infusion broth with adding glucose to the concentration of 0.2% with a titre of 4-5 bln. microbial cells in 1 cm³. Then the rabbits are infected with a hemorrhagic disease virus at min. activity 10³ LD₅₀/cm³ and the fallen rabbits' liver is used to prepare 10-15% suspension. The pathogen cultures are inactivated separately in formalin to the final concentration of no more than 0.4% at temperature 37°C for no more than 4 days; the inactivated culture mixture is added with an adjuvant which is presented by an aluminium hydroxide gel in the amount of 20% to the volume; the end product is thoroughly mixed before packaging.

EFFECT: presented invention enables producing the safe, storage-stable high-immunogenic associated salmonellosis, escherichiosis and rabbit viral hemorrhagic disease vaccine which may be used in biopreparation engineering.

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