FIELD: medicine.
SUBSTANCE: invention describes construction of attenuated microorganisms Clostridium perfringens for immune defense of domestic animals and poultry against Clostridia infections. The attenuated microorganisms are constructed by integrating a nucleic acid molecule coding a substantially non-toxic mutein of Clostridium perfringens alpha toxin, into a chromosome of the attenuated microorganism of the same genus and are able to express the substantially non-toxic alpha toxin. The expressed alpha toxin represents a deletion mutein with its amino acid sequence is lacking in at least nine sequential amino-acid residues, including His68. A version of the attenuated microorganism Clostridium perfringens constructed by the similar method is a microorganism additionally containing at least one gene coding a polypeptide differed from the polypeptide of Clostridium perfringens. The microorganism expresses both the mutein of the alpha toxin, and the polypeptide which can be of a nonbacterial origin, particularly the avian or mammal polypeptide.
EFFECT: attenuated microorganism may be used for preparing vaccines of wide spectrum of action.
13 cl, 7 dwg, 3 tbl, 5 ex
