FIELD: medicine.
SUBSTANCE: invention may be used for human genotype determination by 5382 gene BRCA1 (5382insC) position polymorphism. The method consists in using allele-specific primers with real-time PCR values recording with using fluorescence marked probes. A reaction mixture contains the primers different for each allele, the primer and the probe common for two alleles, and also the common fluorescent probe.
EFFECT: invention provides cutting expenses for genetic researches and making them more available due to the use of standard equipment and only one marked probe.
2 dwg
