FIELD: medicine.
SUBSTANCE: claimed method belongs to medicine, biology and biotechnology fields and concerns definition of human genotype on the basis of polymorphism in interleukin-1-alpha gene in position 889(C/T). The method involves use of allele-specific primers with polymerase chain reaction directly in the course of reaction with the help of fluorescent marked samples, the reaction mix including primers for a site with desirable replacement, different for each allele, and primer with sample common to each couple of alleles.
EFFECT: streamlining of method.
2 cl, 1 dwg
