FIELD: medicine.
SUBSTANCE: invention belongs to medicine, biology and biotechnology and concerns a method of human genotype definition on the basis of interleukin gene 6, position 174(G/C) polymorphism. The method involves use of allele-specific primers with polymerase chain reaction result registration directly in the course of reaction with the help of fluorescent-labeled samples, the reaction mix including primers for a site with desirable replacement, different for each allele, and primer with sample common to each couple of alleles.
EFFECT: streamlining of the method.
1 dwg
Authors
Dates
2008-08-27—Published
2006-10-11—Filed