FIELD: medicine.
SUBSTANCE: method for in vitro recovery of experimental tuberculous granulomas in culture involves induction of granulomatous inflammation by infecting experimental animals with BCG mycobacteria, recovery and mechanical disintegration of spleen tissues containing granulomas over a period adequate to form them in an animal's body, homogenisation of spleen tissue in a solution by agitation, purification of the homogenate from coarse tissue fragments by spontaneous deposition in a culture medium, removal of the deposits, recovery of the granulomas from a supernatant, washing in a new portion of the culture medium by centrifugation at acceleration 15-28 g at least three times and transfer of the deposited granuloma in the culture medium.
EFFECT: effective granuloma integrity and higher purity of the recovered granulomas.
4 cl, 3 ex
