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(19)

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2 447 419

(13)

(51)

IPC

G01N1/00(2006-01-01)

G01N33/48(2006-01-01)

C12N1/20(2006-01-01)

C12R1/32(2006-01-01)

(21) (22)

Application

2010129634/05, 2010-07-15

(24)

Start date

2010-07-15

(22)

Actual filing date

2010-07-15

(45)

Published

2012-04-10

(72)

Inventor

Arkhipov Sergej AlekseevichShkurupij Vjacheslav Alekseevich

(73)

Holder

Uchrezhdenie Rossijskoj Akademii Meditsinskikh Nauk Nauchnyj Tsentr Klinicheskoj I Ehksperimental'Noj Meditsiny Sibirskogo Otdelenija Ramn So Ramn)

METHOD FOR in vitro RECOVERY OF EXPERIMENTAL TUBERCULOUS GRANULOMA IN CULTURES Russian patent published in 2012 - IPC G01N1/00 G01N33/48 C12N1/20 C12R1/32

Abstract RU 2447419 C2

FIELD: medicine.

SUBSTANCE: method for in vitro recovery of experimental tuberculous granulomas in culture involves induction of granulomatous inflammation by infecting experimental animals with BCG mycobacteria, recovery and mechanical disintegration of spleen tissues containing granulomas over a period adequate to form them in an animal's body, homogenisation of spleen tissue in a solution by agitation, purification of the homogenate from coarse tissue fragments by spontaneous deposition in a culture medium, removal of the deposits, recovery of the granulomas from a supernatant, washing in a new portion of the culture medium by centrifugation at acceleration 15-28 g at least three times and transfer of the deposited granuloma in the culture medium.

EFFECT: effective granuloma integrity and higher purity of the recovered granulomas.

4 cl, 3 ex

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RU 2 447 419 C2

Authors

Arkhipov Sergej Alekseevich

Shkurupij Vjacheslav Alekseevich

Dates

2012-04-10—Published

2010-07-15—Filed