DIAGNOSTIC TECHNIQUE FOR TUBERCULOSIS Russian patent published in 2014 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to medical diagnostics, and describes a diagnostic technique by immunohistochemical examination and detection of the Mycobacterium tuberculosis antigen expression. The method is characterised by examining a biological material applied on a slide by a modified immunohistochemical method and involves the following stages: preparing the biological material: smears/scrapes, packed cells of biological fluids to be fixed in acetone; preparing with 1% potassium bichromate; preparing with 3% hydrogen peroxide; applying monoclonal antibodies to Mycobacterium tuberculosis antigens dissolved 1:20; incubating at 3-5 degrees Celsius for 12 hours; applying EnVision detection system and incubating at room temperature; applying diaminobenzidine; counterstaining with haematoxyline; enclosing into a balm or other medium, microscopic examination, diagnosing the disease if observing the Mycobacterium tuberculosis antigen.

EFFECT: technique provides higher accuracy of detection of the Mycobacterium tuberculosis antigen, cost reduction of diagnosing and applicability in practical healthcare.

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