FIELD: medicine.
SUBSTANCE: there is constructed a recombinant plasmid DNA pAP271 containing a rhFVII protein gene, a MAR matrix attachment region of an avian lysozyme gene, CMV virus transcription amplifier, a promoter of translational factor of human EF-1α elongation, an internal site of translational initiation of encephalomyocarditis IRES virus, a mouse DHFR gene, a SV40 virus polyadenylation signal, a cartridge comprising all elements required for aminoglycoside-3'-phosphotransferase (APH) gene expression, a cartridge for expression in bacterial cells of β-lactamase gene, as well as unique recognition sites of the following restriction endonucleases: Agel (850 base pairs), BbvCI (1657 base pairs), BmgBI (4202 base pairs), BssHII (6672 base pairs), Eco47III (11269 base pairs), EcoRI (10929 base pairs), EcoRV (11863 base pairs), Fsel (1455 base pairs), NotI (4812 base pairs), RsrII (6790 base pairs), Sbfl (2330 base pairs), Sfil (6027 base pairs), Tthllll (6390 base pairs), Xcml (2404 base pairs).
EFFECT: presented invention provides producing stably high-yield recombinant human blood coagulability factor VII.
2 cl, 4 dwg, 2 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pAK380 CODING POLYPEPTIDE OF RECOMBINANT FACTOR IX OF HUMAN BLOOD COAGULABILITY, LINE OF CELLS Cricetulus griseus CHO, 1E6-PRODUCER OF RECOMBINANT FACTOR IX OF HUMAN BLOOD COAGULABILITY, AND METHOD FOR OBTAINING POLYPEPTIDE HAVING ACTIVITY OF RECOMBINANT FACTOR IX |
2012 |
- Grigor'Eva Ol'Ga Vasil'Evna
- Zaval'Nyj Mikhail Aleksandrovich
- Karpov Andrej Pavlovich
- Petrov Andrej Vladimirovich
- Fabrichnyj Igor' Pavlovich
- Shuster Aleksandr Mikhajlovich
|
RU2500816C1 |
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2012 |
- Grigor'Eva Ol'Ga Vasil'Evna
- Zaval'Nyj Mikhail Aleksandrovich
- Karpov Andrej Pavlovich
- Petrov Andrej Vladimirovich
- Fabrichnyj Igor' Pavlovich
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|
RU2500818C1 |
| RECOMBINANT PLASMID DNA, ENCODING HUMAN FACTOR VII PROTEIN SEQUENCE, CELL LINE BHK/F7, TRANSFORMED WITH PLASMID DNA |
2006 |
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- Miroshnikov Anatolij Ivanovich
- Gabibov Aleksandr Gabibovich
- Vorob'Ev Ivan Ivanovich
- Ponomarenko Natal'Ja Aleksandrovna
- Shamborant Ol'Ga Georgievna
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- Mart'Janov Vitalij Afanas'Evich
|
RU2337965C2 |
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2014 |
- Kovnir Sergej Vladimirovich
- Orlova Nadezhda Aleksandrovna
- Vorobev Ivan Ivanovich
- Khodak Yuliya Vladimirovna
- Dronina Mariya Alekseevna
- Smirnov Ivan Vitalevich
- Ponomarenko Natalya Aleksandrovna
- Skryabin Konstantin Georgievich
- Gabibov Aleksandr Gabibovich
|
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2009 |
- Orlova Nadezhda Aleksandrovna
- Kovnir Sergej Vladimirovich
- Vorob'Ev Ivan Ivanovich
- Jur'Ev Andrej Serafimovich
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|
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2013 |
- Orlova Nadezhda Aleksandrovna
- Kovnir Sergej Vladimirovich
- Vorob'Ev Ivan Ivanovich
- Vorob'Ev Andrej Ivanovich
|
RU2561466C2 |
| EXPRESSION PLASMID DNA p6E-tTF CODING EXTRACELLULAR AND TRANSMEMBRANE DOMAINS OF HUMAN TISSUE FACTOR AND E.coli BL21 [DE3]/p6E-tTF STRAIN - PRODUCER OF RECOMBINANT HUMAN TISSUE FACTOR |
2008 |
- Orlova Nadezhda Aleksandrovna
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|
RU2426780C2 |
| RECOMBINANT PLASMID DNA pBK415 CODING POLYPEPTIDE OF RECOMBINANT TISSULAR ACTIVATOR OF PLASMINOGEN OF HUMAN BEING, CELL LINE Cricetulus griseus CHO 1F8-PRODUCER OF RECOMBINANT TISSULAR ACTIVATOR OF PLASMINOGEN OF HUMAN BEING, AND METHOD FOR OBTAINING AND SEPARATION OF POLYPEPTIDE HAVING ACTIVITY OF TISSULAR ACTIVATOR OF PLASMINOGEN |
2012 |
- Grigor'Eva Ol'Ga Vasil'Evna
- Zaval'Nyj Mikhail Aleksandrovich
- Karpov Andrej Pavlovich
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|
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| RECOMBINANT PLASMID PET21-TF7, PROVIDING SYNTHESIS OF MODIFIED TISSUE FACTOR, AND ESCHERICHIA COLI BL21 (DE3) PET21-TF7 STRAIN - PRODUCER OF RECOMBINANT HUMAN TISSUE FACTOR |
2020 |
- Shcherbakov Dmitrij Nikolaevich
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- Koval Anatolij Dmitrievich
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|
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2003 |
- Gabibov A.G.
- Ponomarenko N.A.
- Vorob'Ev I.I.
- Demin A.V.
- Mart'Janov V.A.
- Shuster A.M.
- Bairamashvili D.I.
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|
RU2260049C2 |
