FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to recombinant production of coagulation factors, and can be used for expression of human coagulation factor IX (hFIX). Method comprises constructing plasmid for expression of hFIX in CHO cells, consisting of field of replication start OF plasmid pUC, open reading frame of beta-lactamase (bla) and procariotic gene promoter bla; section of a terminal repetition EBVTR, functional promoter and first of elongation factor 1 alpha Chinese hamster, flanked 5' untranslated region of said gene; open reading frame gene coding hFIX; internal site IRES EMCV; open reading frame DHFR expressed in bicystronic mRNA together with gene coding hFIX; and functional terminator gene elongation factor 1 alpha Chinese hamster, flanked 3' untranslated region of said gene.
EFFECT: higher efficiency of product hFIX.
15 cl, 3 dwg, 3 tbl, 7 ex
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