METHOD OF PRODUCING Escherichia coli VL-613 BASED SYMBIOTIC PREPARATION Russian patent published in 2012 - IPC C12N1/20 C12R1/19 

FIELD: chemistry.

SUBSTANCE: method of producing an Escherichia coli VL-613 based symbiotic preparation involves sowing, culturing bacteria in a liquid medium based on Hottinger broth, concentrating the bacterial suspension and then mixing with a stabiliser, packing and lyophilising the preparation. During the process of culturing Escherichia coli VL-613, redox potential of the bacterial culture is reduced to minus (80-100) mV right after sowing. Before the end of the culturing process, pO₂ is kept at 20±5% of saturation of air with oxygen and pH is kept in the 7.0-7.4 range. Glucose is fed in doses until achieving concentration of 0.1-0.2% while limiting growth of Escherichia coli VL-613 with glucose. The culturing process is 4-6 hours long. The preparation contains 80-85% living cells and enables to completely replace synthetic lysine in complete diets when growing broilers.

EFFECT: high quality and stability of the Escherichia coli VL-613 based symbiotic preparation.

3 tbl, 5 ex