FIELD: biotechnology.
SUBSTANCE: method for obtaining a symbiotic preparation involves seeding with Escherichia coli strain VL-613 a nutrient medium based on Hottinger's digest. Concentration of the bacterial suspension is carried out, followed by mixing with the protective medium in a predetermined ratio, followed by packaging in vials. In this case oxidation-reduction potential of the culture liquid is maintained for 75 min – (-160) – (-60) mV, the dissolved oxygen partial pressure in the culture liquid is 15 ± 5 % of the oxygen saturation of the air.
EFFECT: invention allows to shorten the duration of cultivation and to increase the accumulation of viable microbial cells.
1 cl, 4 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING Escherichia coli VL-613 BASED SYMBIOTIC PREPARATION | 2010 |
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