FIELD: medicine.
SUBSTANCE: newborn rabbits' pancreases are placed in a salt solution with an antibiotic at temperature 4-10°C and grounded microfragments. Further they are incubated in a free medium from serum at first incubation temperature 36.6 to 37°C, during a first incubation period with periodic replacement of a serum-free medium and removal of spontaneously collapsed undesired cells until at least 80% of the remained cells represent beta cells. It is followed by incubation in the serum-free medium periodically replaced at second incubation temperature 22 to 29°C, during a second incubation period until at least 78-90 % of the remained cells represent beta cells.
EFFECT: invention allows producing beta cells applicable for transplantation to patients with diabetes for stimulating natural insulin development.
4 cl, 40 tbl, 14 ex
