FIELD: medicine.
SUBSTANCE: invention describes a method of screening estimation of the humal functional body condition, involving the collection of exhaled moisture condensate (expirate), the preparation of a biosensor - luminescent lyophilised bacteria 'Ecolum', the addition of the expirate 0.5 cm3 to the biosensor 0.5 cm3, the 15-minute exposition, the measurement of luminescent emission of mixed bacterial suspension and condensate for 1000 sec, the recording of its maximum (Iop, imp/sec.), the comparison of said value with an analogue parameter Ik, imp/sec., derived by introducing distilled water in a bioluminometer tray instead of condensate in equal proportions, the determination of a coefficient K as the Iop/Ik relation which has a value of K=1 enables to state an optimal oxidative status; the value K>1 shows the prevailing pro-oxidative processes (a high level of formation of oxygen radicals, an insufficient level of protection against their damaging action), the value K<1 provides stating active antioxidant processes, sufficient reserves of protective mechanisms.
EFFECT: simplification, higher productivity and profitability of the method of the estimation of the functional body condition allowing detecting the oxidative status deviations.
3 ex
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Authors
Dates
2012-07-10—Published
2011-06-01—Filed