METHOD OF BIOLOGICAL ASSESSMENT OF OSTEOINDUCING EFFECT OF BLOOD PLASMA PROTEINS Russian patent published in 2012 - IPC A61B10/00 G09B23/28 

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to traumatology and orthopedics. In order to carry out biological assessment of osteoinducing effect of blood plasma proteins modified autoplasm is obtained from human blood. Modified autoplasm is tested on three laboratory animals, which are preliminarily subjected to shin bone fracture, with further, 48 hours later, intraperitoneal introduction of modified autoplasm in volume 0.02 ml. After that, 72 hours after introduction of modified autoplasm, blood is sampled from said animals, with determination of alkali phosphotase activity in its serum. If alkali phosphotase activity in serum of at least two animals is twice or more times higher relative to upper boundary of norm, characteristic of selected animal species, conclusion about presence of osteoinducing effect of tested blood serum proteins is made. If alkali phosphotase activity increases two times and more relative to upper boundary of norm only in one animal, conclusion about absence of osteoinducing effect of tested blood serum proteins is made.

EFFECT: method makes it possible to assess osteoinducing effect of preparation of blood autoplasm proteins, used for stimulation of bone reparation in patients with delayed osteogenesis.

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