FIELD: medicine.
SUBSTANCE: method for commercial production of thrombin provides preparing a raw material, producing a complex of proteins containing prothrombin, activating prothrombin to be transformed in thrombin, purifying by dialysis that is followed by lyophilisation of a thrombin solution. The raw material is added with a heparin solution at 50-800 IU per the raw material 1 l. If the raw material is presented by human blood plasma or Cohn fraction II+III of human blood plasma, the beginning of prothrombin activation is followed by the incubation of the thrombin suspension at 14-18 h, addition of polyethylene glycol 6000 in the amount of 0.75-1.25 % of thrombin suspension weight, reduction of the pH value of the thrombin suspension to 5.25-5.75 by adding 10% or 20% acetic acid. If the raw material is presented by cattle blood plasma, 10 minutes after the beginning of prothrombin activation every 15 minutes for 40-60 minutes distilled water 200 ml containing 20% acetic acid 2 ml; it is followed by adding polyethylene glycol 6000 0.75-1.25 % of thrombin suspension weight, reducing the pH value of the thrombin suspension to 5.25-5.75 by adding 10% or 20% acetic acid. The thrombin yield makes 40-60 thousand NIH units of thrombin activity from 1 l of blood plasma.
EFFECT: higher thrombin yield and improved storage stability of thrombin.
2 cl, 3 tbl
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Authors
Dates
2012-07-27—Published
2010-06-09—Filed