FIELD: medicine.
SUBSTANCE: method involves isolation, culture and replication of avian embryo stem cells in a complete culture medium containing all growth factors in the presence of an inactivated feeder layer, and added with animal serum; separation of the growth factors, serum and feeder layer; production of the non-adhesive embryo stem cells; proliferation of the produced cells in a suspension in the form of cell balls; infection of said cells with said virus; addition of the second serum-free medium to the cell culture; further culture of the infected cells for virus replication and collection of said virus.
EFFECT: invention is applicable for preparing vaccines and particularly enables producing viral strains which cannot be adequately growth in eggs.
38 cl, 23 dwg, 4 ex
