METHOD OF CARRYING OUT PCR-RFLP FOR GENOTYPING CATTLE ON ALLELES A AND K OF GENE DGAT1 Russian patent published in 2014 - IPC C12N15/06 C12Q1/68 

FIELD: veterinary medicine.

SUBSTANCE: method of carrying out PCR-RFLP for genotyping cattle on alleles A and K of gene DGAT1, which is different from the nearest prototype [1] in that at the stage of PCR the other sequences of the oligonucleotide primers: DGAT1-1: 5'- CCGCTTGCTCGTAGCTTTCGAAGGTAACGC-3' (SEQ ID NO:1): DGAT1-2: 5'-CCGCTTGCTCGTAGCTTTGGCAGGTAACAA-3' (SEQ ID NO:2): DGAT1-3: 5'-AGGATCCTCACCGCGGTAGGTCAGG-3' (SEQ ID NO:3) are used, and at the stage of RFLP the other restriction endonuclease - TaqI is used, with the generation of the genotype-specific fragments: the genotype AA=82/18 bp, the genotype KK=100 bp and the genotype AA=100/82/18 bp (Figures 1 and 2).

EFFECT: development of an efficient method of genotyping the cattle on DGAT1-gene based on PCR-RFLP analysis.

2 dwg, 2 tbl