FIELD: medicine.
SUBSTANCE: adherent fraction of mononuclear cells (MNC) is recovered from peripheral blood of patients suffering colorectal cancer and cultivated in the medium of certain composition for producing immature dendritic cells (DC). In the process of cultivation, the latter are added with a tumour antigen (autologous tumour cell lysate), and then rhTNF-α for maturation of the immature DC. The produced mature antigen-activated DC are cultivated with the non-adherent fraction of the MNC in the presence of the proinflammatory cytokines rhIL-12 and rhIL-18.
EFFECT: use of the method of generating the antigen-specific cytotoxic cells with anticancer activity provides high cytotoxicity of anticancer cells, and as a consequence the efficiency of a T-helper type 1 immune response on colorectal cancer antigens.
1 tbl
| Title | Year | Author | Number |
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| GENERATION METHOD OF ANTIGEN-SPECIFIC CYTOTOXIC CELLS WITH ACTIVITY AGAINST OVARIAN CARCINOMA CELLS | 2012 |
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| METHOD OF GENERATING ANTIGEN-SPECIFIC CYTOTOXIC CELLS WITH ANTITUMOUR ACTIVITY IN BREAST CANCER | 2013 |
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| METHOD FOR OBTAINING ANTIGEN SPECIFIC CYTOTOXIC CELLS WITH ANTI-TUMOR CYTOTOXIC ACTIVITY AGAINST CELLS OF NON-SMALL CELLS LUNG CANCER | 2016 |
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| RECOMBINANT PLASMID DNA pCI-UB-POLYEPI CONTAINING EPITOPES OF TUMOUR-ASSOCIATED ANTIGENS FOR COLORECTAL CANCER AND METHOD OF ITS APPLICATION FOR STIMULATION OF SPECIFIC ANTITUMOUR IMMUNE RESPONSE AGAINST COLORECTAL CANCER CELLS | 2012 |
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