IMMUNOTHERAPY OF BREAST CANCER USING ANTIGEN-ACTIVATED DENDRITIC CELLS Russian patent published in 2018 - IPC C12N5/10 

FIELD: medicine.

SUBSTANCE: invention relates to immunology and biotechnology. Invention provides a method of immunotherapy of breast cancer using antigen-activated dendritic cells, including the isolation of mononuclear cells from the patient's peripheral blood, the separation of cells into adherent and non-adherent fractions, the addition of growth factors to the sticking fraction of MNC, loading of immature dendritic cells with lysate antigens of autologous tumor cells of breast cancer in vitro, stimulation of maturation of dendritic cells, co-cultivation of dendritic cells with non-adherent lymphocytes in a ratio of 1:10 with the addition of IL-12 and IL-18, the addition to the sticking fraction of mononuclear cells growth factors are carried out for 72 hours, the dendritic cells are loaded with tumor lysate antigens for 24 hours, the maturation of the dendritic cells is stimulated for 48 hours, then the markers are evaluated for expression on the surface of mature dendritic cells, obtained by co-culturing for 96 hours of mature dendritic cells and lymphocytes of the non-stick fraction of mononuclear cells, are frozen, followed by defrosting and fractionation, wherein the fractionation of thawed cells and their cultivation is carried out for 2–4 hours immediately prior to injection, whereby the cells are divided into adherent and non-adherent fraction, and then enriched with dendritic cells, the adhering fraction is administered to the patient subcutaneously, and activated non-sticking lymphocytes with increased cytotoxic cell content and T-helper type 1 are administered intravenously to the patient on a background of dexamethasone premedication, at least 3–4 courses within 3–4 months, with a total number of injected cells of at least 100 million.

EFFECT: invention allows to provide protection against the formation of distant metastases and stimulates a specific cytotoxic response against the existing ones.

1 cl, 7 dwg