METHOD FOR ENZYMATIC PRODUCTION OF 2-HYDROXY-2-METHYL CARBOXYLIC ACIDS Russian patent published in 2012 - IPC C12P7/42 C12P7/52 C12N9/90 C12N15/61 

FIELD: chemistry.

SUBSTANCE: invention relates to a method for the enzymatic production of 2-hydroxy-2-methyl carboxylic acids from 3-hydroxy carboxylic acids. The disclosed method involves synthesis of 3-hydroxy carboxylic acid in an aqueous reaction solution which contains a unit, having 3-hydroxy carboxylic acid CoA-mutase activity and having both 3-hydroxy-carbonyl-CoA ester-producing and 3-hydroxy-carbonyl-CoA ester-isomerising activity. That unit is selected from a group comprising an isolated cobalamin-dependent mutase, an enzyme or a system of enzymes exhibiting 3-hydroxy-carbonyl-CoA ester, a microorganism or crude extract thereof, adding to said reaction solution, incubation, and then obtaining, appropriately, converted 2-hydroxy-2-methyl carboxylic acid in form of an acid or in form of salts thereof.

EFFECT: method is safe for the environment and has low power consumption.

24 cl, 2 dwg, 2 tbl, 3 ex