APPLICATION OF PISUM SATIVUM KAURENOXIDASE FOR HIGHLY EFFICIENT PRODUCTION OF REBAUDIOSIDES Russian patent published in 2023 - IPC C12N15/81 A23L2/60 C12N9/02 

FIELD: biotechnology.

SUBSTANCE: invention represents compositions and methods for more efficient production of steviol glycosides in the host cell. In some embodiments, the host cell is genetically modified to contain a heterologous nucleotide sequence encoding Pisum sativum kaurene oxidase or a kaurene oxidase variant. In some embodiments, the host cell also contains one or more heterologous nucleotide sequences encoding pathway enzymes capable of producing steviol glycosides in the host cell.

EFFECT: compositions and methods described herein form an efficient method for the heterologous production of steviol glycosides, including but not limited to rebaudioside D and rebaudioside M.

38 cl, 6 dwg, 3 tbl, 6 ex