FIELD: medicine.
SUBSTANCE: invention may be used for target modification of a duplex DNA (double straight DNA) sequence. What is presented is a donor oligonucleotide a sequence of which is complementary along its whole length to the modified acceptor DNA with the exception of one nucleotide forming a mispairing with the modified duplex DNA sequence describing the expected substitute. The oligonucleotide under the invention comprises two modified nucleotide which represent LNA, and spaced at one nucleotide from a mispairing point from both sides.
EFFECT: what is described is a method for effective modification of the duplex DNA sequence with the use of the new oligonucleotide; conditions and means required for implementation thereof are disclosed.
14 cl, 3 dwg, 1 ex
