FIELD: physics.
SUBSTANCE: method involves obtaining a genome sample containing a certain amount of duplex nucleic acids which contain target nucleotide sequences. A hybrid mixture containing the genome sample, probes, hybridisation-promoting agents and labels is prepared. The hybridisation mixture containing complexes of duplex target nucleotide sequences, probes and labels is incubated. The incubated mixture is exposed to radiation capable of stimulating energy radiation of at least some of the labels. Fluorescence data are used to establish whether probe nucleotide sequences are ideally complementary to target nucleotide sequences in order to detect presence of that nucleotide sequence in the genome sample. The method is realised without denaturing duplex nucleic acids and without amplifying duplex nucleic acids via a polymerase chain reaction (PCR), wherein the ratio of the number of probes to the number of duplex nucleic acids ranges from 3.2 × 109 to 1012.
EFFECT: invention enables to detect nucleotide polymorphism in a genome target without target amplification.
53 cl, 5 dwg, 8 tbl, 8 ex
