FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, particularly to detection of a target nucleic acid sequence by a PCE-SH (PTO cleavage and extension-dependent signalling oligonucleotide hybridisation) assay. Present invention does not use probes to be hybridised with target nucleic acid sequences for providing target signals. Present invention uses probes (signalling oligonucleotides) to be hybridised with extended strand formed in a target-dependent manner in which extended strand is synthesised using templating oligonucleotide (CTO) artificially selected as templates.
EFFECT: disclosed is a method of detecting a target nucleic acid sequence by PCE-SH assay.
31 cl, 16 dwg, 4 ex
| Title |
Year |
Author |
Number |
| DETECTION OF NUCLEOTIDE VARIATIONS IN NUCLEIC ACID SEQUENCE-TARGETS IN THE ANALYSIS WITH THE CLEAVAGE AND EXTENSION OF THE PROBING AND TAGGING OLIGONUCLEOTIDE (PTO) |
2013 |
- Chun Dzhon Joon
- Li Yang Dzho
|
RU2620955C2 |
| DETECTION OF NUCLEIC ACID TARGET SEQUENCE IN ANALYSIS WITHOUT HYBRIDIZATION, DEPENDING ON CLEAVAGE AND ELONGATION OF THE PROBING AND LABELING OLIGONUCLEOTIDE (PTO) |
2013 |
- Chun Dzhon Joon
- Li Yang Dzho
|
RU2620958C2 |
| DETERMINATION OF TARGET NUCLEIC ACID SEQUENCES BY SPLITTING OLIGONUCLEOTIDE PROBE AND HYBRIDISATION |
2012 |
- Chun Dzhon Joon
- Li Yang Dzho
|
RU2588457C2 |
| DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION ASSAY |
2012 |
- Chun Dzhon Joon
- Li Jang Dzho
|
RU2566562C2 |
| TARGET-DISCRIMINATING PROBE, METHOD OF CONSTRUCTING THEREOF AND METHOD OF DETECTING NUCLEIC ACID TARGET-SEQUENCE (VERSIONS) |
2010 |
- Chun, Dzhon Joon
- Khvan, In Taehk
- Leeh, San Kil
|
RU2542478C2 |
| TARGET DETECTION WITH TSG-PRIMER |
2010 |
- Chun, Dzhon Joon
- Khvan, In Taehk
|
RU2551321C2 |
| DETECTION OF NUCLEOTIDE TARGET SEQUENCES USING DIFFERENT DETECTION TEMPERATURES |
2014 |
- Chun Dzhon Joon
- Li Eng Cho
|
RU2780587C2 |
| DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING DIFFERENT DETECTION TEMPERATURES |
2014 |
- Chun Dzhon Joon
- Li Eng Cho
|
RU2678403C2 |
| PLANT TRANSFORMANTS OF PV-ZMIR13 (MON863) MAIZE AND COMPOSITIONS AND METHODS OF IDENTIFYING THEM |
2003 |
- Kavat Trejsi A.
- Kumb Timoti R.
- Dzhonson Skott K.
|
RU2352638C2 |
| DETECTION OF PLURALITY OF TARGET NUCLEIC ACIDS USING PLURALITY OF DETECTION TEMPERATURES |
2022 |
- Lee, Han Bit
- Kim, Jeong Woo
- Kim, Hyeon Be
|
RU2821888C2 |
