FIELD: medicine.
SUBSTANCE: there are presented versions of recombinant virulent polypeptides and nucleic molecules coding them with the sequences disclosed in the description, used to prepare a vaccine composition for A/E-pathogen. What is described is a vaccine composition for inducing a response on A/E-pathogen in animals containing said nucleic acid molecule; a cell culture supernatant containing said polypeptide; and/or a bacterium or its preparation having a mutation in its genome in said nucleic acid molecule inducing virulence attenuation, or having nleA gene deletion in a combination with a physiologically acceptable carrier. What is presented is a method for virulence attenuation of A/E-pathogen, involving nleA gene deletion in A/E-pathogen or deletion of one or more said nucleic acids in the A/E-pathogen genome.
EFFECT: invention provides producing the effective vaccine.
24 cl, 2 tbl, 27 dwg, 9 ex
