METHOD FOR STEROID PROFILING IN DOPE TEST OF SPORTSMEN Russian patent published in 2012 - IPC G01N33/50 G01N33/52 G01N33/74 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely sports medicine, and may be used for steroid profiling in dope test of sportsmen. That is ensured by urine sample hydrolysis by a mixture of two enzymes: β-glucuronidase E.coli and arylsulphatase H.pomatia in volume ratio 1:1 to 1:3 in the presence of a citrated buffer solution and an internal standard. The hydrolysate is separated, derivatised that is followed by chromatic/mass-spectral analysis of the sample and recording of the derived results and stating the presence of endogenic steroids (ES). The ES value is determined in a sportsman for at least 10-15 times every 10-12 days. The analysis results are used to set minimum and maximum values of each of the derived ES. Said values are accepted to be a confidence range, and a steroid profile of the sportsman is drawn as a set of confidence ranges of all found ES.

EFFECT: invention provides steroid profiling, extending a variety of the stated ES and ensuring a higher degree of reliability of stating the ES.

2 cl, 12 dwg, 5 tbl, 5 ex