FIELD: medicine.
SUBSTANCE: invention relates to genetic engineering, biochemistry, biotechnology and immunology. Constructed is recombinant plasmid pBi121-ESAT6-CFP10-gIFN, with size 15796 bp, containing the following constructive elements: fragment of DNA of vector plasmid pBi121 with size 14726 bp; Xbal - BamHI fragment, coding chimeric gene, consisting of two genes esat6 and cfp10 M. tuberculosis and human γ-interferon; 5'UTR region from genome of tobacco etch virus; double 35S CaMV promoter from genome of cauliflower mosaic virus; 3'UTR region from genome of cauliflower mosaic virus. Constructed plasmid ensures transfer of nucleotide sequence of chimeric gene cfp10-esat6-gIFN into genome DNA of plants.
EFFECT: invention can be applied for obtaining anti-tuberculosis vaccines of novel generation.
3 dwg, 2 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pTB232, WHICH CODES HYBRID POLYPEPTIDE GST-CFP10 WITH PROPERTIES OF SPECIES-SPECIFIC MYCOBACTERIAL ANTIGEN CFP10, RECOMBINANT STRAIN OF BACTERIA Escherichia coli-PRODUCER OF HYBRID POLYPEPTIDE GST-CFP10 AND RECOMBINANT POLYPEPTIDE GST-CFP10 |
2008 |
- Tumanov Jurij Vasil'Evich
- Smirnova Ol'Ga Jur'Evna
- Boldyrev Aleksandr Nikolaevich
- Tat'Kov Sergej Ivanovich
|
RU2381274C2 |
| METHOD FOR PREDICTING TUBERCULOUS INFECTION, RECOMBINANT PLASMID DNA CODING THE SYNTHESIS OF CFP10-ESAT6 HYBRID PROTEIN OUT OF MYCOBACTERIUM TUBERCULOSIS, THE METHOD OF ITS OBTAINING, CFP10-ESAT6 HYBRID PROTEIN AND ITS APPLICATION |
2003 |
- Katlinskij Anton Vikent'Evich
- Kiselev Vsevolod Ivanovich
- Pal'Tsev Mikhail Aleksandrovich
- Perel'Man Mikhail Izrailevich
- Severin Evgenij Sergeevich
|
RU2277540C2 |
| RECOMBINANT PLASMID pESAT6-CFP10-DBD, RECOMBINANT STRAIN Escherichia coli M15 [pREP4, pESAT6-CFP10-DBD], METHOD FOR PREPARING, IMMOBILISING, CONCENTRATING AND PURIFYING RECOMBINANT PROTEIN ESAT6-CFP10-DBD ON DEXTRAN, RECOMBINANT PROTEIN ESAT6-CFP10-DBD AND IMMUNOGENIC COMPOSITION CONTAINING PROTEIN ESAT6-CFP10-DBD |
2013 |
- Tkachuk Artem Petrovich
- Ljashchuk Aleksandr Mikhajlovich
- Aksenova Ekaterina Ivanovna
- Poletaeva Nina Nikolaevna
- Galushkina Zoja Mikhajlovna
- Sergienko Ol'Ga Vasil'Evna
- Kondrat'Eva Tat'Jana Konstantinovna
- Apt Aleksandr Solomonovich
- Erokhin Vladislav Vsevolodovich
- Karjagina-Zhulina Anna Stanislavovna
- Lunin Vladimir Glebovich
- Gintsburg Aleksandr Leonidovich
|
RU2539026C1 |
| RECOMBINANT PLASMID DNA pBi101-IL18 ENCODING HUMAN INTERLEUKIN-18 SYNTHESIS IN TRANSGENIC PLANTS |
2005 |
- Turchinovich Andrej Alekseevich
- Dejneko Elena Viktorovna
- Filipenko Maksim Leonidovich
- Shumnyj Vladimir Konstantinovich
- Khrapov Evgenij Aleksandrovich
- Sennikov Sergej Vital'Evich
|
RU2302460C1 |
| RECOMBINANT PLASMID DNA PG11 ENCODING SYNTHESIS OF HUMAN BETA-INTERFERON IN TRANSGENIC PLANTS AND A METHOD OF PRODUCING TRANSGENIC PLANTS SHOWING RESISTANCE TO VIRAL INFECTION |
1994 |
- Rivkin M.I.
- Dejneko E.V.
- Komarova M.L.
- Shumnyj V.K.
|
RU2103361C1 |
| RECOMBINANT PLASMIDE ENCODING HYBRID GST-ESAT-6 POLYPEPTIDE HAVING ACTIVITIES OF SPECIES-SPECIFIC MYCOBACTERIAL ESAT-6 ANTIGEN, RECOMBINANT ESCHERICHIA COLI STRAIN AS PRODUCER OF HYBRID GST-ESAT-6 POLYPEPTIDE AND RECOMBINANT GST-ESAT-6 POLYPEPTIDE |
2004 |
- Tumanov Jurij Vasil'Evich
- Smirnova Ol'Ga Jur'Evna
- Boldyrev Aleksandr Nikolaevich
- Tat'Kov Sergej Ivanovich
|
RU2282661C2 |
| METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON CFP10-DBD HYBRID PROTEIN AND DEXTRANE; pCFP10-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pCFP10-DBD] STRAIN; CFP10-DBD CHIMERIC PROTEIN AND THEIR APPLICATION |
2013 |
- Tkachuk Artem Petrovich
- Ljashchuk Aleksandr Mikhajlovich
- Aksenova Ekaterina Ivanovna
- Poletaeva Nina Nikolaevna
- Galushkina Zoja Mikhajlovna
- Sergienko Ol'Ga Vasil'Evna
- Kondrat'Eva Tat'Jana Konstantinovna
- Apt Aleksandr Solomonovich
- Erokhin Vladislav Vsevolodovich
- Karjagina-Zhulina Anna Stanislavovna
- Lunin Vladimir Glebovich
- Gintsburg Aleksandr Leonidovich
|
RU2546875C1 |
| RECOMBINANT PLASMID DNA pTB323 CODING HYBRID POLYPEPTIDE CST-DELTAMPT64 WITH PROPERTIES OF SPECIES-SPECIFIC MYCOBACTERIAL ANTIGEN MPT64 (MPB64), RECOMBINANT ESCHERICHIA COLI BACTERIAL STRAIN - PRODUCER OF HYBRID POLYPEPTIDE CST-DELTAMPT64 AND RECOMBINANT POLYPEPTIDE CST-DELTAMPT64 |
2011 |
- Tumanov Jurij Vasil'Evich
- Smirnova Ol'Ga Jur'Evna
- Boldyrev Aleksandr Nikolaevich
|
RU2458130C1 |
| HYBRID PROTEIN CFP10-ESAT6, INDUCING REACTION OF HYPERSENSITIVITY OF DELAYED TYPE WITH RESPECT TO M.Tuberculosis, CODING IT CHIMERIC NUCLEIC ACID AND RECOMBINANT PLASMID EXPRESSION VECTOR, CONTAINING IT, METHOD OF OBTAINING HYBRID PROTEIN AND BASED ON IT DOSED MEDICINAL FORM FOR INTRACUTANEOUS INJECTION |
2008 |
- Kiselev Vsevolod Ivanovich
- Pal'Tsev Mikhail Aleksandrovich
|
RU2360926C9 |
| RECOMBINANT PLASMID DNA PG21 ENCODING HUMAN β-INTERFERON IN PLANTS |
1993 |
|
RU2035513C1 |
