METHOD OF DETECTING VIABLE PROBIOTIC MICROORGANISMS IN IN VITRO CONDITIONS, IMITATING DIGESTION PROCESS IN HUMANS Russian patent published in 2012 - IPC C12Q1/04 A61K35/74 

FIELD: medicine.

SUBSTANCE: in in vitro conditions, imitating digestion process in humans quantities of live microorganisms at the beginning and at the end of experiment are determined and their numeric values are compared. Conclusion is made on the basis of the results of comparison after incubation of probiotic microorganisms during 4 h in acidic model medium with acidin-pepsin by inoculation of microorganism suspension on dense nutritional medium. Grown colonies are counted and number of viable microorganisms is determined. Remaining suspension is separated from incubation medium, alkaline model medium with pansinorm forte 20000 is added to sediment in volume, analogous to volume of acidic model medium. Sediment is resuspended and suspension is incubatred during 12 hours, remaining number of viable microorganisms is determined by inoculation on a dense nutritional medium and counting grown colonies.

EFFECT: increased efficiency and accuracy of method and possible therapeutic-preventive efficiency of medication, administered to patient with intestinal disbacteriosis.

5 cl, 4 ex