FIELD: medicine.
SUBSTANCE: method of determination includes: sorption in microboard holes of IgAl-protease,introduction into microboard holes of samples, which contain functionally determinable active C1 inhibitor, carrying out incubation, during which covalent binding of C1 inhibitor with IgAl-protease takes place, determination of amount of bound in holes C1 inhibitor by means of conjugate of antibodies to C1 inhibitor with enzyme and chromogenic substrate for said enzyme. Method is based on ability of functionally active C1 inhibitor to bind covalently with IgAl-protease. Kit contains flat-bottomed microboard with sorbed IgAl-protease preparation, conjugate of enzyme with antibodies to human C1 inhibitor, substrate buffer of said enzyme and standard for active C1 inhibitor.
EFFECT: group of inventions ensures elaboration of simple method and kit for determination of functional activity of C1 inhibitor and studying its inhibition.
2 cl, 1 dwg
