FIELD: medicine.
SUBSTANCE: invention represents a method of evaluating functional activity of Clr2s2 subcomponents of the first component of a human complement involving sorption in microplate wells of a classical complement cascade, introduction of a solution containing porpoise serum and sodium ethylene diamine tetraacetate; then incubation and drying of the plate is followed by introducing the analysed sample containing the Clr2s2 complex of the human complement of unknown activity, and a pseudoglobulin fraction of human blood serum prepared by distilled water dialysis (R1), and also a buffer solution containing calcium and magnesium ions, incubation, and after washing and drying of the plate, introduction of a enzyme conjugate with human C3 component and substratum antibodies into the wells, calculation of Clr2s2 subcomponent activity by an amount of the produced enzyme reaction product, differing by the fact that the classical complement cascade is derinate, and also a kit for functional activity assay on Clr2s2 subcomponents of the first component of the human complement.
EFFECT: invention provides developing the method of functional activity assay on Clr2s2 complex of the human complement showing good reproducibility.
2 ex, 1 dwg
