FIELD: medicine.
SUBSTANCE: pharmaceutical preparation derinate representing sodium deoxyribonucleate is sorbed in microplate wells. Then, an analysed sample containing a component C3 of unknown activity and a solution of reagent R3 (component C3 activity deficient human blood serum) is added in the wells. It is followed with incubation, and after washing and drying of the dish, a conjugate of enzyme with component C3 antibodies and a substratum of this enzyme are added into the wells. The component C3 activity is evaluated by the amount of the prepared product of enzymatic reaction. A kit comprises a flat-bottomed microplate with sorbed derinate, the conjugate of enzyme with human complement component C3 antibodies, the reagent R3 (component C3 activity deficient human blood serum) and the substrate buffer.
EFFECT: method allows the reliable and high sensitive component C3 test.
1 dwg, 3 ex
