FIELD: biotechnology.
SUBSTANCE: method provides mixing a suspension of the microorganism with the substrate (10% solution of glucose) and a hydrogen acceptor, which is used as a methylene blue colorant followed by measuring the concentration of methylene blue during the enzymatic reaction. According to the rate of change of the concentration of methylene blue in the linear phase of the enzymatic reaction the dehydrogenase activity is determined.
EFFECT: invention enables to reduce time of determining the dehydrogenase activity of microorganisms and to improve the quality of its determination.
1 dwg
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